How To Reconstitute Peptides. Mixing Made Easy!

How To Reconstitute Peptides. Mixing Made Easy!

*This post contains affiliate links*

When it comes to getting a peptide or water soluble substance there’s a few issues.

When people have peptides, you notice something odd.

You have this weird, disc shaped powder.

Not an oil or water that’s ready for use immediately.

As a result, I get a LOT of questions or concerns from people.

Just to name a few common questions I see:

  • What do I mix it with?
  • How do I know how much solution to use?
  • How do I calculate how much of the product I have per use?
  • Is there any best practices to keep it shelf stable longer?

I’ll address all of these questions and more below.

Also, In the post I have linked a Peptide Calculator.

As always, please remember to read the plethora of disclaimers

“What do I mix my peptides with?”

9/10 the answer is “Bacteriostatic Water.”

Photo presenting a vial of bacteriostatic water for reconstitution.
Bacteriostatic water: literally just looks like regular… water.

Bacteriostatic water is sometimes colloquially called “Bac Water.”

Sometimes people will receive items in kits with “Sterile water for Reconstitution.”

Other times people will see “Acetic acid for reconstitution”

I’ll talk a bit more about each of these solutions briefly, but 99% of the time.. people use bac water.

why do people use Sterile water for reconstitution?

Sterile water as a solvent for your peptides is typically only in circumstances of SINGLE-use.

This is because properly sealed and stored sterile water is free of bacteria or adulterants.

However; once the vial is used, it now has the opportunity for bacteria or other agents to adulterate the water.

Sterile water is just pure water without any agents to help prevent bacterial growth.

However; Bacteriostatic water or acetic acid which work in slightly different ways to prevent bacterial growth.

Reconstitution with either of those is for multiple use products.

why do people use bacteriostatic water for reconstitution

This is typically what you find from medical facilities selling bacterostatic water

Bacteriostatic water is a solution that typically contains 0.9% “Benzyl Alcohol” per solution as well as sterile water.

The Benzyl Alcohol works to prevent bacterial growth inside of the solution, due to the alcohol.

This also means that the solution created may have a longer shelf life because it won’t be easily degraded by adulterants.

Bacteriostatic water can overtime become worthless, especially after use.

A general rule of thumb people use: after 4 weeks, pitch the opened/used vial.

This is because of the potency from the Benzyl Alcohol is severely impaired from the exposure of other agents.

What do people use Acetic Acid For Reconstitution?

Acetic Acid is a solution that contains 10ml sterile water that is typically diluted with 6mg of acetic acid.

Glacial Acetic Acid is pretty much purified Acetic Acid (it’s less than 1% water content)

It is much more expensive and not necessary.

Typically Acetic Acid is used for more PH sensitive Peptides: (like IGF1-LR3)

The general train of thought is that the alcohol used in bacteriostatic water will denaturize the peptide proteins faster.

As a result people aim to use Acetic acid for their suspension as it also has a 4 week shelf life.

With acetic acid via Intramuscular or Subcutaneous (subQ) use have issues with a burning sensation.

This is where people will use the acetic acid to reconstitute first, then sterile water or bac water to backfill the syringe before administering.

This dilution occurs AFTER suspension and ONLY in the insulin syringe, not in the vial.

Calculating how much to use

I used to have people check out PinfoBox for peptide calculations, however

I have recently found an OUTSTANDING WEBSITE.

Unlike Pinfobox, they currently do not charge you a monthly fee.

No, I am not affiliated with them, but this is insanely beneficial:

You can literally pick your syringe size, amount of solution used and the amount you want per injection.

if you don’t have access to the website, here’s an example of the math behind figuring out your injection.

Example: (how much bacteriostatic water to mix with 5mg of bpc-157)

lets say I have a 5mg vial of BPC-157.

Now, if i want to get 250mcg per shot, how would I do that?

I have two of the THREE pieces of information needed.

lets assume i have 1CC (1ml) insulin syringe.

with that I can do an easy calculation.

1000mcg= 1mg

with that, I can do basic multiplication:

(1mg= 1000mcg) x 5 (the amount of BPC-157 in the vial)

5mg= 5000mcg

we know my syringe can hold 1cc/1ml of solution, so that’s not useful YET.

If I add ONE CC (ML) of bacteriostatic water to my BPC-157, I know I’ll have 5000mcg per vial

Now, since I’ve only added ONE ML of Bac water, If I have a 1ML Syringe that will fill it up ENTIRELY.

Each syringe has the amount of “Units” used. (also called TIC marks)

This is used to denote how much you are going to be injecting.

You can typically find 100 TIC mark 1 ML syringes easily on Amazon.

They’re going to have every 10 tics denoting a TENTH of a ML.

Now, if I draw to 10 units/ the 10-tick mark I’m using one-tenth of the solution.

That means my injection is 500mcg.

That’s more than I wanted.

So that means I have TWO OPTIONS:

Add 1 more ML of bacterostatic water to get 250mcg per injection

OR just draw in-between half of the 0 and 10 tic marker.

For 5mg of BPC-157 you need 2ml of bacteriostatic water.

A short reference on common dosages for peptides

At the Request of a reader; here is a table with the peptide name, ML of water, and typical dosage utilized.

Peptide NameML for ReconstitutionTypical Dose (1ml 100 tic Syringe )
HCG (5000 iu)1ML Bac Water1000 IU (20 tic marks)
BPC-157(5mg)2ML Bac Water250-500mcg (10-20tic marks)
TB-500 (5mg)1ML Bac Water2.5mg (50 tic marks)
Follistatin (1mg)1ML Bac Water100mcg (10 tic marks)
HGH (10 iu Vial)1ML Bac Water1-4 IU (10-40 tic marks)
HGH-Frag (5mg)1ML Bac Water500mcg (10 tic marks)
GHRP-2 (5mg)2ML Bac Water100mcg (4 tic marks)
GHRP-6 (5mg)2ML Bac Water100mcg (4 tic marks)
IGF1-LR3 (1mg)1ML Acetic Acid100mcg (10 tic marks)

Best practices for longer lasting products.

I talked about this in my previous post where I talk about supplements and their “expiration” dates.

But, if you don’t want to read all of that follow these instructions.

  • Make sure to wipe down the tops of your vials with an Isopropyl alcohol pad before and after use.
  • Do not adulterate your peptides with anything other than your preferred solution.(Bacteriostatic/Sterile water/Acetic acid)
  • Refrigerate after reconstitution (keep away from food products)
  • Do not let product sit out in direct sunlight
  • Do NOT freeze the solution after mixing
  • Keep the product out of temperature sensitive climates (don’t let the vial sit out after drawing)

If you have any additional questions or concerns, just let me know.


Links to items discussed for peptide reconstitution:

Syringes mentioned in blogpost (Click here)

Link to inexpensive bacteriostatic water (Click here)

Link to Acetic Acid (click here)

Additionally, if you use swisschems for your peptides, save 10% on your order using code VG11!

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